Levofloxacin eye drops are light yellow to light yellow or light yellow green clear liquid. It is applicable to eye diseases such as blepharitis, eyelid adenitis, dacryocystitis, conjunctivitis, tarsal plate adenitis, keratitis caused by Staphylococcus, Streptococcus, pneumococcus, Micrococcus and Enterococcus.

In order to prevent the eye drops from being polluted by microorganisms in the process of use and preservation, an appropriate amount of bacteriostatic agents are often added. Therefore, the rational use and quality control of bacteriostatic agents have become one of the key problems to ensure the safety and effectiveness of eye drops. Welch Materials brings you the content determination scheme of bacteriostatic agent in levofloxacin eye drops.

Chromatographic conditions

Column: Xtimate®C18 (4.6 × 250mm,5 μ m)
Mobile phase: aqueous phase (4ml triethylamine and 7ml phosphoric acid are added into 1000ml water): acetonitrile = 35:65;
Wavelength: 214nm;
Temperature: 30 ℃;
Flow rate: 1.0ml/min;
Injection volume: 20 μ L;

Spectra and data

  1. Blank solvent

2. Benzalkonium bromide reference solution


3. Test solution

Full scale diagram

Partial enlarged view



Using Welch Xtimate ® C18(4.6 × 250mm,5 μ m) The chromatographic column can meet the detection requirements under this chromatographic condition

Product information

P/NProduct nameSpecification
00101-21043Xtimate® C184.6×250mm,5μm
00802-02201Welchrom ® Syringe FilterImported NY, 13mm*0.22μm, 100PK
00821-32291Welchrom ®Cap + septaPre slit red Teflon / White Silicone septa, 9mm blue short neck
open top cap, dia 6mm 100pk
00821-40927 Welchrom ® Sample Vials2ml transparent short neck sample vials
with label 11.6 * 32mm glass 100pk

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