Scope of application ✦

It is suitable for the detection of amproline residues in bovine muscle, liver, kidney and fat (the matrix used in this experiment is bovine fat)

Reference standard: “GB 31613.1-2021 National Food Safety Standard – Determination of Amproline Residues in Bovine Edible Tissue – Liquid Chromatography-Tandem Mass Spectrometry and High Performance Liquid Chromatography Second Method High Performance Liquid Chromatography”

Extraction steps ✦

Weigh 5 g of the sample, add 10 mL of acetonitrile, extract by ultrasonic for 5 min, centrifuge at 5000 r/min for 5 min, take the clear liquid, add 10 mL of acetonitrile to the residue and repeat the extraction, combine the acetonitrile layers, add 5 mL of n-propanol, 50 ℃ rotary evaporation to dryness, add 20 mL of phosphate buffer, to be purified.

Purify ✦

SPE cartridge: Welchrom® BRP, 500mg/6mL.

Activation: 5mL methanol, 5mL water, discard;

Loading: take 10mL sample and discard;

Rinse: 5mL water, discard;

Elution: 4mL mobile phase, to be measured.

Chromatographic conditions ✦

Column: UTisil® XB-C18, 4.6×250mm, 5μm.

Mobile phase: sodium heptane sulfonate solution: methanol: acetonitrile = 60:35:5;

Flow rate: 1.0mL/min;

Column temperature: 30℃;

Injection volume: 20μL;

Wavelength: 267nm

Spectra and Data ✦

  1. Amprolium 0.1mg/L spectrum

2.Blank chromatogram of bovine fat sample

3.Beef fat sample 40μg/kg spiked atlas

Increased recovery rate

NameSpike level
μg/kg
The average recovery rateRSD %
(n=2)
Amphetine40772

Product Information ✦

Aarticle numberNameSpecification
00522-20006SPE cartridgeWelchrom® BRP,
500mg/6mL, 30pk
00837-0500650mL screw
Conical centrifuge tubes
Welchrom® Centrifuge Tubes Disposable centrifuge tubes,
Flat Lid, Conical Bottom, RCF12000xg,
Bag, Unsterilized, 50mL, 50/pk
00837-0500215mL screw
Conical centrifuge tubes
Welchrom® Centrifuge Tubes Disposable centrifuge tubes,
Flat Lid, Conical Bottom, RCF12000xg,
Bag, Unsterilized, 15mL, 50/pk
00824-31001Solid Phase Extraction
device
Welch solid phase extraction unit,
12-bit square cylinder
00821-32291Cover + gasketWelchrom® Pre-Slit Red Teflon/
White silicone septa, 9mm blue short thread
Opening cover center hole 6mm 100pk
00821-40927VialWelchrom® 2mL Clear Short Thread Wide Mouth
Sample bottle with writing place 11.6*32mm
First-class hydrolyzed glass 100pk
00201-31043Liquid analytical columnUltisil® XB-C18
4.6*250mm, 5μm

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