ColumnXtimate® C18 (4.6×250mm, 5μm)
Mobile Phase0.05mol/L potassium dihydrogen phosphate solution (adjust pH to 5.0 with
2mol/L potassium hydroxide solution) / acetonitrile=97.5/2.5
Detection254nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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