Mobile Phase
| Time（min） | A% | B% |
| 0 | 35 | 65 |
| 35 | 35 | 65 |
| 60 | 80 | 20 |
| 70 | 85 | 15 |
| 80 | 35 | 65 |
| Column | Welch Ultisil PFP 4.6×250mm，5μm |
| Mobile Phase | A: acetonitrile B: water |
| Detector | 227nm |
| Temperature | 30℃ |
| Flow Rate | 2.6 ml/min |
| Injection Volume | 10µl |
If you have any problem or require further information, please contact info@welchmat.com.
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