Determination of Alanylglutamine Related Substances by Ultisil ODS-3

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ColumnUltisil® ODS-3（4.6×250mm，5μm）
Mobile Phase0.01mol/L phosphate buffer (adjust pH to 2.3 with phosphoric
acid)/acetonitrile=96/4
Detection200nm
Temperature10℃
Flow Rate1.0ml/min
Injection Volume20μL
NoteSystem equilibration takes a long time

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