System adaptability solution

ColumnUltisil®AQ-C18(
4.6×200mm,5μm)
Mobile PhaseDilute phosphoric acid solution: take 11.5G phosphoric acid, add
88.5ml water, and shake well; Measure 23.5ml, add water to dilute to 1000ml, shake well and filter.

Dilute phosphoric acid solution / acetonitrile = 94 / 6
Detection209nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul
Note/

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