Blank solution diagram
| Column | Ultisil® PFP (4.6×250mm,5μm) |
| Detection | 258nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | / |
Mobile Phase
Phosphate buffer solution: take 0.02mol/l potassium dihydrogen phosphate and 0.02mol/l potassium dihydrogen phosphate, mix them 1 / 1, adjust the pH to 6.5 with 20% phosphoric acid solution, mix them, and filter them.
| Time(min） | Phosphate buffer (%) | Acetonitrile (%) |
| 0 | 100 | 0 |
| 10 | 100 | 0 |
| 30 | 70 | 30 |
| 35 | 70 | 30 |
| 36 | 100 | 30 |
| 45 | 100 | 0 |
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