Reference solution

ColumnUltisil®ODS-3(
4.6×250mm,5μm)
Mobile PhaseMobile phase: mix 1ml formic acid, 160ml acetonitrile and 840ml water, degassing by ultrasound.

Acetonitrile / water / formic acid = 16 / 84 / 0.1
Detection250nm
Temperature40℃
Flow Rate1.0ml/min
Injection Volume10ul
Note\

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