Range of Application
It is suitable for the determination of acetaminophen residue in muscle, liver and kidney tissue of cattle, sheep and pig (pork was selected as matrix in this experiment).
Purification Steps
SPE column: Welchrom®BRP column, specification: 60 mg/3mL.
Activation: 3 mL methanol, 3 mL water, discard
Sample loading: load all the samples, discard
Flushing: 3 mL water, 3 mL 5% methanol, discard
Elution: 4 mL methanol, blow the eluent to dry at 40℃ nitrogen, and add it to 1 mL with the mobile phase
Chromatographic Conditions
Column: UItisil®XB-C18,4.6×250 mm, 5μm.
Mobile phase: methanol: 0.05 mol/L ammonium acetate solution=2:8
Flow rate: 1 mL/min
Column temperature: 30℃
Injection volume: 50 μL
Wavelength: 250 nm
Chromatogram or Spike Recovery Rate
Chromatogram of 0.1mg/L Acetaminophen
| Name | Rt | Area | Height | Relative peak area | Tailing factor | Plate |
| _ | min | mAU*min | mAU | % | EP | (USP) |
| Acetaminophen | 7.313 | 0.303 | 2.140 | 100 | 1.01 | 16844 |
Figure 2. Blank Chromatogram of Pork Sample
Figure 3. Chromatogram of Pork Sample at 0.02mg/kg
Table 1 Spike Recovery Rate
| Name | Rt mg/kg | Average Recovery Rate % | RSD% （n=2） |
| Acetaminophen | 0.02 | 97 | 4.70 |
Ordering Information
| P/N | Product | Specification |
| 00522-20009 | SPE column | Welchrom® BRP, 60mg/3mL, 50pk |
| 00837-05006 | 50mL centrifuge tube with screw mouth and apical bottom | Disposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization |
| 00837-05002 | 15mL centrifuge tube with screw mouth and apical bottom | Disposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/pk without sterilization |
| 00824-31001 | Welch SPE manifold | 12 ports |
| 00821-32291 | Caps and septa | Pre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk |
| 00821-40927 | Vials | 2ml wide opening short screw-thread vial with write-on spot, clear, 11.6*32mm, 100/pk |
| 00201-31043 | HPLC Column | Ultisil® XB-C18, 4.6×250mm, 5μm |
If you have any problem or require further information, please contact info@welchmat.com.
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