Determination of Acetaminophen in Pork

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Range of Application

It is suitable for the determination of acetaminophen residue in muscle, liver and kidney tissue of cattle, sheep and pig (pork was selected as matrix in this experiment).

Purification Steps

SPE column: Welchrom®BRP column, specification: 60 mg/3mL.

Activation: 3 mL methanol, 3 mL water, discard

Sample loading: load all the samples, discard

Flushing: 3 mL water, 3 mL 5% methanol, discard

Elution: 4 mL methanol, blow the eluent to dry at 40℃ nitrogen, and add it to 1 mL with the mobile phase

Chromatographic Conditions

Column: UItisil®XB-C18,4.6×250 mm, 5μm.

Mobile phase: methanol: 0.05 mol/L ammonium acetate solution=2:8

Flow rate: 1 mL/min

Column temperature: 30℃

Injection volume: 50 μL

Wavelength: 250 nm

Chromatogram or Spike Recovery Rate

Chromatogram of 0.1mg/L Acetaminophen

NameRtAreaHeightRelative peak areaTailing factorPlate
 _minmAU*minmAU%EP(USP)
Acetaminophen7.3130.3032.1401001.0116844

Figure 2. Blank Chromatogram of Pork Sample

Figure 3. Chromatogram of Pork Sample at 0.02mg/kg

Table 1 Spike Recovery Rate

NameRt
mg/kg
Average Recovery Rate
%
RSD%
(n=2)
Acetaminophen0.02974.70

Ordering Information

P/NProductSpecification
00522-20009SPE columnWelchrom® BRP, 60mg/3mL, 50pk
00837-0500650mL centrifuge tube with screw mouth and apical bottomDisposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/ pk without sterilization
00837-0500215mL centrifuge tube with screw mouth and apical bottomDisposable centrifuge tube with flat cap, and conical bottom, RCF12000xg, 15 mL, 50/pk without sterilization
00824-31001Welch SPE manifold12 ports
00821-32291Caps and septaPre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk    
00821-40927Vials2ml wide opening short screw-thread vial with write-on spot, clear, 11.6*32mm, 100/pk  
00201-31043HPLC ColumnUltisil® XB-C18, 4.6×250mm, 5μm

If you have any problem or require further information, please contact info@welchmat.com.


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