ColumnXtimate® C18(4.6×250mm,5μm)
Mobile PhasePhosphate buffer (weigh 1.19 g of sodium dihydrogen phosphate and 1 g of sodium pentanesulfonate, dissolve in 500 ml of water, and adjust the pH to 3.0 with phosphoric acid)/acetonitrile=92/8
Detection230nm
Temperature30℃
Flow Rate1.0ml/min
Injection volume5μL
NoteSystem equilibration takes a long time

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