4-Piperidinic acid single standard solution
| Column | Topsil ® Silica(4.6×250mm，5μm) |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.1ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
| Time(min） | Mobile phase A (%) | Acetonitrile (%) |
| 0 | 20 | 80 |
| 8 | 20 | 80 |
| 15 | 40 | 60 |
| 20 | 40 | 60 |
| 21 | 20 | 80 |
| 35 | 20 | 80 |
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