
Column | Ultisil®XS-C18(4.6*250 mm,5 μm) |
Mobile Phase | See the table below for details |
Detection | 221nm |
Temperature | 25℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 10 μL |
Mobile Phase:
Time (min) | Acetonitrile/% | Water/% |
0 | 35 | 65 |
30 | 35 | 65 |
35 | 90 | 10 |
45 | 90 | 10 |
46 | 35 | 65 |
56 | 35 | 65 |
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