PBS blank solution
| Column | Xtimate®C18 (4.6*250 mm,5 μm) |
| Detection | 260nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | / |
Mobile Phase
A: Buffer salt solution B: methanol
| Time(min） | Mobile phase A (%) | Mobile phase B (%) |
| 0 | 100 | 0 |
| 5 | 100 | 0 |
| 15 | 10 | 90 |
| 20 | 10 | 90 |
| 21 | 100 | 0 |
| 35 | 100 | 0 |
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