| Column | Xtimate® C18(4.6×250 mm,5 μm) |
| Mobile Phase | See the table below for details |
| Detection | 205nm |
| Temperature | Room temperature |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 5 μL |
Mobile Phase:
| Time (min) | Acetonitrile:Methanol=7:3% | 0.1% Phosphoric acid% |
| 0 | 0 | 100 |
| 5 | 0 | 100 |
| 20 | 30 | 70 |
| 25 | 30 | 70 |
| 30 | 0 | 100 |
| 40 | 0 | 100 |
If you have any problem or require further information, please contact info@welchmat.com.
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