Reference solution
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | Water-acetonitrile-methanol（75:5:20） |
| Detector | 269nm |
| Temperature | 35℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Notes | Pressure is relatively small at 35℃ than that of room temperature |
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