Reference solution

ColumnWelch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
Mobile PhaseWater-acetonitrile-methanol(75:5:20)
Detector269nm
Temperature35℃
Flow Rate1.0ml/min
Injection Volume20ul
NotesPressure is relatively small at 35℃ than that of room temperature

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