Crude extract separation and purification by Ultisil LP-C18

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ColumnUltisil®LP-C18(21.2×250 mm,5 μm)
Mobile PhaseA: Acetonitrile
B: Water
Running gradient: 0~16min 100%A 17min 60%A
Detection260nm
Temperature30℃
Flow Rate15.0 ml/min
Injection Volume5000 μL

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Categories: Applications Info

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