Content Determination of Related Substance of Gamithromycin on Xtimate C18

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Solution of mixed standards

ColumnWelch Xtimate® C18(4.6×250mm,5um)
Wavelength215nm
Temperature35℃
Flow Rate1.0mL/min
Injection Volume10μL

Mobile Phase

TimeMobile phase AMobile phase B
07030
304060
404060
40.17030
557030

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