Solution of mixed standards
| Column | Welch Xtimate® C18(4.6×250mm，5um) |
| Wavelength | 215nm |
| Temperature | 35℃ |
| Flow Rate | 1.0mL/min |
| Injection Volume | 10μL |
Mobile Phase
| Time | Mobile phase A | Mobile phase B |
| 0 | 70 | 30 |
| 30 | 40 | 60 |
| 40 | 40 | 60 |
| 40.1 | 70 | 30 |
| 55 | 70 | 30 |
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