Content Determination of HD127-093-10 on Ultisil ALK C18

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Test solution

ColumnWelch Ultisil® ALK C18(4.6×250mm,5um)
Mobile PhaseDiammonium hydrogen phosphate solution/acetonitrile =100/3
Wavelength210nm
Temperature25℃
Flow Rate1.5 mL/min
Injection Volume20 μl

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