Content Determination of Andrographis Paniculata on Blossmate C18
Reference solution
| Column | Welch Blossmate® C18（4.6×250mm，5μm) |
| Wavelength | 205 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 5 μL |
Mobile Phase
| Time | Water (%) | Acetonitrile (%) |
| 0 | 80 | 20 |
| 15 | 75 | 25 |
| 30 | 72 | 28 |
| 60 | 60 | 40 |
| 65 | 15 | 85 |
| 65.1 | 80 | 20 |
| 75 | 80 | 20 |
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