Content Determination of Andrographis Paniculata on Blossmate C18

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Reference solution

ColumnWelch Blossmate® C18(4.6×250mm,5μm)
Wavelength205 nm
Temperature30℃
Flow Rate1.0 mL/min
Injection Volume5 μL

Mobile Phase

TimeWater (%)Acetonitrile (%)
08020
157525
307228
606040
651585
65.18020
758020

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