Application Range

It is suitable for the determination of acetylsalicylic acid in facial paste, cream and lotion cosmetics for whitening and moisturizing purposes (the substrate used here is lotion).

Reference standard: Detection of acetylsalicylic acid in imported cosmetics SN/T 2290-2009

Extraction Steps

Weigh 1g sample accurately(0.01g) in 15 ml centrifuge tube with plug. Then add 10 ml methanol and mix well. Extract by ultrasound for 10 min, centrifuge at 8000r/min for 5 min, take the supernatant to filter through 0.22um filter membrane to be tested.

Chromatographic Condition

Column: Ultisil@XB-C18, 4.6*250mm, 5um

Mobile phase: acetonitrile: tetrahydrofuran: acetic acid: water =20:5:70

Flow rate:1.0mL/min

Column temp: 30℃

Injection volume: 10μL

Wavelength: 303 nm

Chromatogram and Data 

Acetylsalicylic acid 5mg/L

PeakRtAreaHeightAreaTailing factorPlates
Acetylsalicylic Acid14.4110.96893.481000.9816684

Blank cosmetic water sample

Blank cosmetic water sample spiked at 50mg/kg

Spike recovery rate table

NameSpike LevelAverage Recovery RateRSD
 _mg/kg% %(n=4)
Acetylsalicylic Acid501011.62

Ordering Information

00837-0500215mL centrifuge tube with screw mouth and apical bottomDisposable centrifuge tube, flat cap,conical bottom, RCF12000xg,15 mL, 50/ pk without sterilization
00821-32291Caps and septaPre-slit red PTFE/white Silicone septa, 9mm blue short screw-thread polypropylene cap, 6mm centre hole, 100/pk   
00821-40927Vials2ml wide opening short screw-thread vial with write-on spot, clear, 11.6*32mm , 100/pk
00201-31041HPLC ColumnUltisil® XB-C18,4.6×250mm,5μm

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