Organic phase, pH value, injection volume, column temperature, etc.
Mobile phase: methanol: 10mmol/L sodium hexanesulfonate (pH2.8 buffer salt) = 35:65
|Molar concentration ratio of |
aniline to ion-pairing reagent
|Number of theoretical |
|1:2||10167||1.50||Slightly cutting edge|
Conclusion: When designing the method, it is recommended that the ion-pairing reagent concentration is at least 4 times greater than the sample, and the limit ratio is 1:1. If the sample concentration is too much greater than the ion-pairing reagent concentration, double peaks will appear.
Changes in temperature can cause significant changes in relative retention.
Since the column temperature will change the amount of ion-pair reagents adsorbed on the chromatographic column, the selectivity will change due to the difference in the concentration of the ion-pair reagents.
There will be some variance, but since in ion-pair chromatography the column packing materials are partially covered by the ion-pair reagent, switching column types is not the preferred approach.
Buffer salt type
There will be a certain effect, with little impact on selectivity.