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Clozapine on Xtimate C18 USP 40

System suitability solution(Full Scale)

Column Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043)
Detection 257 nm
Temperature 20℃
Flow Rate 1.2 mL/min
Injection Volume 20 µL
Notes A Ghost-buster column should be installed before injector to remove ghost peaks

Mobile Phase

Time(min) Phosphate buffer (%) Acetonitrile/methanol =55/45 (%)
0 80 20
20 20 80
30 10 90
31 80 20
45 80 20