System suitability solution(Full Scale)
| Column | Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043) |
| Detection | 257 nm |
| Temperature | 20℃ |
| Flow Rate | 1.2 mL/min |
| Injection Volume | 20 µL |
| Notes | A Ghost-buster column should be installed before injector to remove ghost peaks |
Mobile Phase
| Time(min) | Phosphate buffer (%) | Acetonitrile/methanol =55/45 (%) |
| 0 | 80 | 20 |
| 20 | 20 | 80 |
| 30 | 10 | 90 |
| 31 | 80 | 20 |
| 45 | 80 | 20 |
If you have any problem or require further information, please contact info@welchmat.com.
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