System adaptive solution

Rt (min)K’Area (mAU*s)Height (mAU)Symmetry FactorWidth (min)Plates ResolutionTailing Factor
ColumnUltisil XB-C18 4.6×250 mm, 5 μm
Detection220 nm
Temperature35°C
Flow Rate1.2 ml/min
Injection Volume50 µl

Mobile Phase

A: 0.05mol/L sodium dihydrogen phosphate (Adjust pH to 4.0 with phosphoric acid)

B: Acetonitrile:Methanol=3:2

Time(min)A%B%
05545
123862
703862
725545
805545

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