System Suitability
| Column | Ultisil XB-C18, 5μm, 4.6×150mm (P/N 00201-31041) |
| Mobile Phase | Methanol:Buffer(adjust pH to 5.7 with 10% phosphate) =70:30 |
| Detection | 215nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
| Note | Adjust mobile phase pH only after methanol and buffer well mixed |
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