Mixed reference solution
| No. | RT | Compound | Area | Height | Area% | Plates （USP） | Resolution （USP） |
Gradient Program
| Time（min） | A% | B% |
| 0 | 95 | 5 |
| 40 | 5 | 95 |
| 41 | 95 | 5 |
| 46 | 95 | 5 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | A：Buffer-90% acetonitrile methanol（92:8） B: Buffer-90% acetonitrile methanol（52:48） |
| Detector | 210nm |
| Temperature | 40℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 50µl |
If you have any problem or require further information, please contact info@welchmat.com.
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