Sample solution
| Column | Welch Xtimate®C18（4.6×250mm，5μm) and Guard column |
| Mobile Phase | 1.7% sodium dioctyl sulfonate methanol/water/acetic acid=700/300/100 |
| Wavelength | 296nm |
| Temperature | 25℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 100 μl |
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