System Suitability Solution
254nm
| Column | Ultisil AQ-C18, 3μm, 4.6×250mm (P/N 00207-21043) |
| Mobile Phase | Acetonitrile:Buffer(6ml 10% C16H37NO, add water to 200ml, adjust pH to 6.5 with phosphoric acid):Water=20:20:60 |
| Detection | 230nm, 254nm |
| Temperature | 40℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
| Note | Column pressure over 250bar |
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