Sample
| Column | Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043 |
| Detection | 225 nm |
| Temperature | 35℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 10 µl |
| Notes | Sensitive to inject volumn and solvent |
Mobile Phase
A: phosphate buffer B: Acetonitrile
| Time (min) | A (%) | B (%) |
| 0 | 90 | 10 |
| 25 | 75 | 25 |
| 40 | 60 | 40 |
| 45 | 90 | 10 |
| 50 | 90 | 10 |
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