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Chenodeoxycholic Acid on Ultisil XB-C18

Reference standard

Rt (min) K’ Area (mAU*s) Height (mAU) Symmetry Factor Width (min) Plates Resolution Tailing Factor
Column Ultisil XB-C18 4.6×250 mm, 5 μm
Mobile Phase Methanol: water=80:20(adjust pH to 3.7 with 10% perchloric acid)
Detection RI detector
Temperature Column:35°C, RI detector: 35°C
Flow Rate 1.0 ml/min
Injection Volume 20 µl