| Column | Welch Xtimate G-10 14x400mm (P/N: 00110-00154) |
| Mobile Phase | A: 0.1 mol/L phosphate buffer with 3.5% ammonium sulfate（pH 7.0) [0. 1 mol/L disodium hydrogen phosphate solution – 0.1 mol/L sodium dihydrogen phosphate solution (61:39)] B: Water |
| Detector | 254 nm |
| Temperature | 25℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 100ul |
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