Mobile Phase(PH 7.0)

ColumnWelch Xtimate SEC 120 7.8x300mm, 5μm  (P/N: 00237-31052)
  Mobile PhasePhosphate buffer (pH 7.0): 0.05mol/L disodium hydrogen phosphate solution -0.05mol/L sodium dihydrogen phosphate solution =61 :39 , adjusted to pH 7.0 with phosphoric acid
Detector254nm
Temperature30℃
Flow Rate0.5ml/min
Injection Volume10ul

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