Content Determination
| Column | Welch Xtimate C18 4.0x125mm,5μm (P/N: 00101-21031) |
| Mobile Phase | Phosphate buffer (take about 800ml 0.05mol/l disodium hydrogen phosphate solution, adjust pH to 7.7 with 0.05mol/l potassium dihydrogen phosphate solution)-acetonitrile= 88:12 |
| Detector | 254nm |
| Temperature | 25℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
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