Content Determination

ColumnWelch Xtimate C18 4.0x125mm,5μm  (P/N: 00101-21031)
  Mobile PhasePhosphate buffer (take about 800ml 0.05mol/l disodium hydrogen phosphate solution, adjust pH to 7.7 with 0.05mol/l potassium dihydrogen phosphate solution)-acetonitrile= 88:12
Detector254nm
Temperature25℃
Flow Rate1ml/min
Injection Volume20ul

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