| Column | Welch Xtimate C18 4.0x125mm,5μm (P/N: 00101-21031) |
| Mobile Phase | Acetonitrile-phosphate buffer solution (0.05 mol/L disodium hydrogen phosphate solution, pH adjusted to 7.7 with 0.05 mol/L potassium dihydrogen phosphate solution) = 12:88; |
| Detector | 254nm |
| Temperature | 25℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
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