Part of the Scale
Gradient Program
| Time(min) | A | B |
| 0 | 86 | 14 |
| 7 | 86 | 14 |
| 9 | 82 | 18 |
| 16 | 82 | 18 |
| 45 | 60 | 40 |
| 50 | 60 | 40 |
| 55 | 86 | 14 |
| 60 | 86 | 14 |
| Column | Xtimate® C18, 3.9×150 mm, 5 μm (P/N: 17101-21032) |
| Mobile Phase | A: phosphate buffer: 7.1g anhydrous disodium hydrogen phosphate was accurately weighed and dissolved with 1000 mL water. After blending, pH value was adjusted to 6.25 with phosphoric acid. B: methanol |
| Detection | 235nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
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