Part of the Scale

Gradient Program

Time(min)AB
08614
78614
98218
168218
456040
506040
558614
608614
ColumnXtimate® C18, 3.9×150 mm, 5 μm (P/N: 17101-21032)
Mobile PhaseA: phosphate buffer: 7.1g anhydrous disodium hydrogen phosphate was accurately weighed and dissolved with 1000 mL water. After blending, pH value was adjusted to 6.25 with phosphoric acid. B: methanol
Detection235nm
Temperature30℃
Flow Rate1.0 mL/min
Injection Volume10 µL

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