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ColumnUltisil® BD-C18,4.6×250mm, 5μm
Mobile Phase(Water: Acetonitrile: 1M acetic acid: Solution A=876:120:2.8:1.2): Acetonitrile= 93:7 Solution A: triethylamine 14mL, glacial acetic acid 5.7mL, dilute with water into 100mL
Detection254 nm
Temperature40 ℃
Flow Rate2.7ml/min
Injection Volume10 µl

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