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Cefdini on Ultisil XB-C18

Solution for related substance and impurities

No. Component Rt (min) Height (uAU) Area (uAU*s) Percentage (%) Plates Resolution Tailing Factor
1 Impurity 1 23.789 87 2279.7 0.043 18590 0.00 0.84
2 Cefdinir 24.824 201417 5340708.9 99.907 19770 1.47 2.19
3 Impurity 2 27.210 76 1882.9 0.035 27210 3.49 0.77
4 E isomer 37.929 84 790.6 0.015 366335 23.54 0.88
Column Ultisil XB-C18 4.6×250 mm, 5 μm (P/N: 00201-31043)
Detection 250 nm
Temperature 45℃
Flow Rate 1.0 mL/min
Injection Volume 10 µL

Mobile Phase

Mobile Phase A (%): Take 10 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 1000ml, adjust pH to 5.5 by phosphoric acid, then add 0.4ml of 0.1M Disodium edetate;

Mobile Phase B (%): Take 5 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 500ml, adjust pH to 5.5 by phosphoric acid, add 300ml of acetonitrile, add 200ml Methanol, then add 0.4ml of 0.1M Disodium edetate;

Time(min) Mobile Phase A (%) Mobile Phase B (%)
0 95 5
2 95 5
22 75 25
32 50 50
37 50 50
38 95 5
48 95 5