Solution for related substance and impurities
| No. | Component | Rt (min) | Height (uAU) | Area (uAU*s) | Percentage (%) | Plates | Resolution | Tailing Factor | |
| 1 | Impurity 1 | 23.789 | 87 | 2279.7 | 0.043 | 18590 | 0.00 | 0.84 | |
| 2 | Cefdinir | 24.824 | 201417 | 5340708.9 | 99.907 | 19770 | 1.47 | 2.19 | |
| 3 | Impurity 2 | 27.210 | 76 | 1882.9 | 0.035 | 27210 | 3.49 | 0.77 | |
| 4 | E isomer | 37.929 | 84 | 790.6 | 0.015 | 366335 | 23.54 | 0.88 |
| Column | Ultisil XB-C18 4.6×250 mm, 5 μm (P/N: 00201-31043) |
| Detection | 250 nm |
| Temperature | 45℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
Mobile Phase
Mobile Phase A (%): Take 10 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 1000ml, adjust pH to 5.5 by phosphoric acid, then add 0.4ml of 0.1M Disodium edetate;
Mobile Phase B (%): Take 5 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 500ml, adjust pH to 5.5 by phosphoric acid, add 300ml of acetonitrile, add 200ml Methanol, then add 0.4ml of 0.1M Disodium edetate;
| Time(min) | Mobile Phase A (%) | Mobile Phase B (%) |
| 0 | 95 | 5 |
| 2 | 95 | 5 |
| 22 | 75 | 25 |
| 32 | 50 | 50 |
| 37 | 50 | 50 |
| 38 | 95 | 5 |
| 48 | 95 | 5 |
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