Cefdini on Ultisil XB-C18

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Solution for related substance and impurities

No.ComponentRt (min)Height (uAU)Area (uAU*s)Percentage (%)PlatesResolutionTailing Factor 
1Impurity 123.789872279.70.043185900.000.84 
2Cefdinir24.8242014175340708.999.907197701.472.19 
3Impurity 227.210761882.90.035272103.490.77 
4E isomer37.92984790.60.01536633523.540.88 
ColumnUltisil XB-C18 4.6×250 mm, 5 μm (P/N: 00201-31043)
Detection250 nm
Temperature45℃
Flow Rate1.0 mL/min
Injection Volume10 µL

Mobile Phase

Mobile Phase A (%): Take 10 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 1000ml, adjust pH to 5.5 by phosphoric acid, then add 0.4ml of 0.1M Disodium edetate;  

Mobile Phase B (%): Take 5 ml of 0.25% concentration Ammonium tetramethyl hydroxide, add water to 500ml, adjust pH to 5.5 by phosphoric acid, add 300ml of acetonitrile, add 200ml Methanol, then add 0.4ml of 0.1M Disodium edetate;  

Time(min)Mobile Phase A (%)Mobile Phase B (%)
0955
2955
227525
325050
375050
38955
48955

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