Impurity A,E
| Column | Ultisil® LP-C8, 5μm, 4.0×250mm PN: 00209-31034 |
| Detection | 220 nm |
| Temperature | 40 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: dipotassium phosphate 1.6895g, dilute into 970mL with water, add 30mL acetonitrile, adjust pH to 2.5 with Phosphate B: dipotassium phosphate 1.0451g, dilute into 600mL with water, add 400mL acetonitrile, adjust pH to 2.5 with Phosphate
| Time (min) | Mobile Phase A (%) | Mobile Phase B (%) |
| 0 | 100 | 0 |
| 30 | 0 | 100 |
| 35 | 0 | 100 |
| 35.01 | 100 | 0 |
| 40 | 100 | 0 |
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