Cefazolin Sodium on Ultisil LP-C8

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Impurity A,E

ColumnUltisil® LP-C8, 5μm, 4.0×250mm    PN: 00209-31034
Detection220 nm
Temperature40 ℃
Flow Rate1 ml/min
Injection Volume20 µl

Mobile Phase

A: dipotassium phosphate 1.6895g, dilute into 970mL with water, add 30mL acetonitrile, adjust pH to 2.5 with Phosphate B: dipotassium phosphate 1.0451g, dilute into 600mL with water, add 400mL acetonitrile, adjust pH to 2.5 with Phosphate

Time (min)Mobile Phase A (%)Mobile Phase B (%)
01000
300100
350100
35.011000
401000

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