Oxygen is a hazardous substance for most of the capillary columns. At room temperature, the column will not be damaged. But as the column temperature increases, the column will be severely damaged. It is important to be noted that polar GC columns will be severely damaged at low oxygen concentrations. Part.1 Apparent phenomena of oxidative damage and influence on analysis ● Influence on column efficiency The Read more…
Superior core-shell column There are mainly two kinds of chromatographic packing materials: silica gel and polymer. Their structural difference makes maintenance and separation results different. Silica based packing materials contain fully porous silica, ethyl bridged hybrid silica, monolithic silica and core-shell silica. Ultra-efficient analytical results of UHPLC can be obtained on a HPLC instrument with a core-shell Read more…
When use the amino((NH2) column for a period of time, do you encounter a decrease in column efficiency and an increase in column pressure？Or abnormal interference peaks and the retention time drifts? Or method can’t be reproduced under certain chromatographic conditions? Or the resolution, plates and tailing factor can’t meet Read more…
The United States Pharmacopoeia (USP) is a recognized statutory public standard setting agency in the United States of America. USP develops quality standards for prescription and OTC medicines, food supplements, and other health care products and works with healthcare organizations to help them to meet standards. For 198 years of existence, Read more…
Today we will bring “Solutions to solve poor reproducibility between batch to batch”. Q How to ensure good reproducibility when a new validated HPLC method is transferred to Q.C. lab? HPLC column’s reproducibility is very important if a method will be used for a long time. It is necessary to confirm that the column is stable supplied, which Read more…