I believe that most laboratory workers have more or less encountered an experimental phenomenon that makes everyone more headaches in daily testing: when we continuously inject samples, the retention time of the sample cannot be reproduced. For retention time skew and retention time fluctuation. Generally, when looking for the cause, Read more…
Using ultra-high-purity silica gel matrix,High-quality chromatographic packing materials produced by unique patented technology Excellent silica gel matrix ensures high mechanical strength of chromatographic packing materials Packing materials will bear high mechanical pressure during filling and separation and purification, so the mechanical strength requirements for packing are usually higher than those Read more…
Gradient elution can be used to deal with samples that cannot be separated by isocratic elution, and the most common factor for using gradients is that multiple components have different retention capabilities, can‘t be fully eluted in an isocratic elution, or can’t achieve satisfactory separation effect in isocratic elution. To Read more…
Gradient elution can be used to deal with samples that cannot be separated by isocratic elution, and the most common factor for using gradients is that multiple components have different retention capabilities, cannot be fully eluted in an isocratic elution, or cannot achieve satisfactory separation effect isocratic in an isocratic Read more…
Principle Affinity chromatography is based on the inherent specific interactions of biomolecules for highly selective sample separation. Specific interactions include the binding of alcohol energy to substrates, inhibitors, coenzymes, etc. Binding of antibodies to antigens: binding of lectins to cell surface antigens and some sugars. The separation principle is shown Read more…
Food safety has always been the most concerned, especially plant food, which contains a large number of pesticide residues, is a headache for many people. GB 23200.113 and GB 23200.121 complement each other, making the detection more convenient and fast, the strongest combination! GB 23200.113 is “National Standard for Food Read more…
After reading the previous article, you have a clear understanding of reversed ion pair chromatography. Today, I will share with you a practical case: Formamide and other 6 substances separation Initial condition Column: Ultisil® LP-C18 (4.6×250mm, 5μm)Mobile phase: 0.1% sevofluoro-butyric acid solution/acetonitrileDetection wavelength: 210nmColumn temperature: 30℃Flow rate: 1mL/minSample size: 10μL Read more…
Effect of solvent The main reason for the prevalence of solvent effect in TCM samples is the solvent. Most TCM components need to be dissolved in pure organic phase or a high proportion of organic phase. However, in liquid phase analysis, due to the need for proper retention time, a Read more…
Gel filtration chromatography is a technique for elution and separation according to the difference of molecular weight of each component in the separated sample by using the action of molecular sieve of gel particles with porous network structure. Today we mainly introduce some important parameters in gel filtration chromatography. Parameters Read more…
Method adjustment Organic phase, pH value, injection volume, column temperature, etc. Injection volume Mobile phase: methanol: 10mmol/L sodium hexanesulfonate (pH2.8 buffer salt) = 35:65 Molar concentration ratio of aniline to ion-pairing reagent Number of theoretical plates Symmetry factor Peak shape 1:8 16689 1.05 Normal 1:4 13786 1.13 Normal 1:2 10167 Read more…