What is Enzyme-Linked ImmunoSorbent Assay (ELISA)? Enzyme-Linked ImmunoSorbent Assay, or ELISA, is an immunoenzymatic technique that emerged following the development of immunofluorescence and radioimmunoassay technologies. Since its inception in the early 1970s, ELISA has experienced rapid growth. ELISA is a rapid detection technique established on the basis of the specific Read more…
Ribonuclease A Ribonuclease A (RNase A), abbreviated as RNase A, is a single-chain polypeptide containing four disulfide bonds, with a molecular weight of approximately 13.7 kDa (amino acid sequence). RNase A functions as an endoribonuclease. Purification Strategy for Ribonuclease through Chromatography Extraction and Crude Purification Protocol We primarily employ the Read more…
In the development of reversed-phase liquid chromatography (RPLC) methods, the selection of chromatographic columns is generally approached through two methods: one based on practical experience and the other rooted in theoretical knowledge, encompassing both the stationary phase and the chemical properties of the molecules. Today, let’s delve into the process Read more…
In the development process of liquid chromatography methods, the chromatographic column has the greatest impact on separation efficiency. Parameters of the chromatographic column include: bonded phase, column length, diameter, particle size, and pore size. Engineers experienced in liquid chromatography surely remember this mnemonic: Now, under the same method conditions, does Read more…
The function of a buffer solution is to maintain the pH of a solution within a certain range, ensuring that the acidity of the test solution conforms to the specified range of the analytical method. In the case of phosphate buffer solution, with a cutoff wavelength below 200nm, it remains Read more…
01 Overcoming the “Neck-Blocking” Issue in Chiral Chromatography Packing As a domestic manufacturer specializing in the independent production of chromatography packing and columns in China, Welch has long been involved in the research and development, as well as the manufacturing, of chiral chromatography packing and columns. We have successfully addressed Read more…
Following the previous article “1Can LP Columns Only Resist Strong Acids?” this article takes a user-centric approach, guiding everyone in a clear and in-depth exploration to understand the manufacturer’s design principles for chromatography columns. Behind it lies many “treasures” — the mysterious and unknown applications. In our experiments, we unexpectedly Read more…
A core-shell chromatography column, renowned for its features of “rapid separation, standard column pressure, and ultra-high column efficiency,” serves as a significant tool to achieve UHPLC (Ultra-High-Performance Liquid Chromatography) effects with regular liquid chromatography. We have steadily expanded its product offerings on the basis of the original six core-shell chromatography Read more…
Given that we are primarily involved in laboratory testing and inspection in our daily professional endeavors, the sight of our fellow professionals during visits to the laboratory engenders a particularly warm sense of camaraderie. It’s only natural that we share a common language in such settings. Upon encountering some notably Read more…
Ultisil® series, as Welch’s flagship product, has been widely used by numerous customers. You may have noticed a subseries under it, called “LP.” If you’ve paid attention to the LP series, your initial impression is likely “resistant to strong acids.” But is LP only about acid resistance? Product information generally Read more…