Column Welch Ultisil®LP-C18（4.6×250mm，5μm) Mobile Phase Time 0.05 mol/L Potassium Acetonitrile (%） Dihydrogen Phosphate(pH6.0)(%)0 98 210 95 520 80 20 21 98 2 30 98 2 ColumnTemperature 30 ℃ Detectionwavelength: 254 nm Flow Rate 0.8 ml/min InjectionVolume 20 μL Note /
Column Welch Ultisil ® C18（4.6×250mm，5μm) Mobile Phase Mobile Phase A: Take 1 mL of triethylamine and 1 mL of trifluoroacetic acid, add them to 1000 mL of purified water, mix well, and degas using ultrasound to obtain the solution.Time(min) Mobile Phase A(%) Acetonitrile (%) 0 75 2520 45 5525 15 Read more…
Column Welch Xtimate® Sugar-H (7.8×300mm, 5μm) Mobile Phase 0.05 mol/L Phosphoric Acid Solution ColumnTemperature 60 ℃ Detectionwavelength 200 nm Flow Rate 0.4 ml/min InjectionVolume 20 μL Note /
Column Welch Xtimate® SEC-300（7.8×300mm，5μm) Mobile Phase 0.1 mol/L sodium nitrate solution (containing 0.02% 2-methyl-4-isothiazolin-3-onebiocide) ColumnTemperature 35 ℃ Detectionwavelength: Differential detector, detector temperature 35℃. Flow Rate 0.6 ml/min InjectionVolume 100 μL Note /
Column Welch WM-InoWAX (30 m×0.32 mm, 0.25 μm) (Cat. No.: 03909-32001) Mobile Phase 0.05 mol/L Phosphoric Acid Solution Column Temperature 145 ℃ Injection 250 ℃ Detection FID 250 ℃ Carrier gas Nitrogen Flow Rate 1.0 ml/min Split ratio 40-1 Injection Volume 2 μL Hydrogen 30 mL/min Air 300 mL/min Note /
Column Welch Ultisil® LP-C18（4.6×250mm，5μm） Mobile Phase Mobile Phase A: Take 100 mL of chromatography-grade acetonitrile and mix with 900 mL of Wahaha water, shake well, and sonicate to remove air bubbles.Mobile Phase B: Take 600 mL of chromatography-grade acetonitrile and mix with 400 mL of Wahaha water, shake well, and Read more…
Column Welch Ultisil ® Amy-D（4.6×150mm，5μm ) Mobile Phase Isobutyl alcohol/ethanol/trifluoroacetic acid=85/15/0.1 ColumnTemperature 25 ℃ Detectionwavelength 250 nm Flow Rate 0.8 ml/min InjectionVolume 20 μL Note /
Column Welch Ultisil ® HILIC Amphion II(4.6×250m) Mobile Phase 0.1% TFA aqueous solution: acetonitrile (containing 0.1% TFA) = 40:6 ColumnTemperature 35 ℃ ELSD Detection 60℃ Flow Rate 1 ml/min InjectionVolume 0.2 μL Note /
Column Welch Ultisil ®AQ-C18（4.6×250mm，5μm） Mobile Phase ColumnTemperature 35 ℃ Detectionwavelength 210nm Flow Rate 1.0 ml/min InjectionVolume 25 μL Note /
Column Welch Ultisil ® XB C18（2.1×50mm，1.8μm） Mobile Phase ColumnTemperature 35 ℃ Flow Rate 0.3 ml/min InjectionVolume 1 μL Note If there is still residual doxycycline after high-concentration injection, it is recommended to increase the elution time of EDTA-2Na. EDTA-2Na cannot be directly introduced into mass spectrometry, so it is necessary Read more…