Column Welch Ultisil ® Amy-D（4.6×150mm，5μm ) Mobile Phase Isobutyl alcohol/ethanol/trifluoroacetic acid=85/15/0.1 ColumnTemperature 25 ℃ Detectionwavelength 250 nm Flow Rate 0.8 ml/min InjectionVolume 20 μL Note /
Column Welch Ultisil ® HILIC Amphion II(4.6×250m) Mobile Phase 0.1% TFA aqueous solution: acetonitrile (containing 0.1% TFA) = 40:6 ColumnTemperature 35 ℃ ELSD Detection 60℃ Flow Rate 1 ml/min InjectionVolume 0.2 μL Note /
Column Welch Ultisil ®AQ-C18（4.6×250mm，5μm） Mobile Phase ColumnTemperature 35 ℃ Detectionwavelength 210nm Flow Rate 1.0 ml/min InjectionVolume 25 μL Note /
Column Welch Ultisil ® XB C18（2.1×50mm，1.8μm） Mobile Phase ColumnTemperature 35 ℃ Flow Rate 0.3 ml/min InjectionVolume 1 μL Note If there is still residual doxycycline after high-concentration injection, it is recommended to increase the elution time of EDTA-2Na. EDTA-2Na cannot be directly introduced into mass spectrometry, so it is necessary Read more…
Column Welch Ultisil ® AQ-C18（4.6×250mm，5μm) Mobile Phase Acetate buffer/acetonitrile=93/7 ColumnTemperature 30 ℃ Detectionwavelength 272nm Flow Rate 1.0 ml/min InjectionVolume 20 μL Note /
Column Welch Ultisil® UHPLC LP-C18 2.1×150mm，1.8μm Mobile Phas 0.1% formic acid aqueous solution: acetonitrile = 1:1 Column Temperature 40 ℃ Flow Rate 0.2 ml/min InjectionVolume 2 μL Note The matrix curve was used in this test
Column Welch Ultisi® XB-C18 (4.6×250 mm, 3 μm) two in series Mobile Phase Acetonitrile with 0.1% TFA Detection UV detector (254nm) Column Temperature 30℃ Flow Rate 0.5 ml/min Injection Volume 20 μL
Column Welch Xtimate Phenyl-Hexyl (4.6×150mm,3μm); Mobile Phase Methanol: ethanol = 80: 20 Column Temperature 15 ℃ Detection wavelength: 275nm Flow Rate 1 ml/min Injection Volume 25 μL Note N/A
Column Welch Ultisil ® Alk-C18 (4.6×250mm, 5μm) Mobile Phase Acetonitrile/water/triethylamine=10/90/1 (pH3.0) Detection wavelength 212m Column Temperature 30℃ Flow Rate 1.0 ml/min Injection Volume 10 μL
Column Welch Ultisil® F-C8（4.6×250mm，5μm) Mobile Phase Phosphoric acid solution / Acetonitrile / Methanol = 78/15/15 Detection wavelength 210 nm Column Temperature 46 ℃ Flow Rate 11.0 ml/min Injection Volume 20 μL