Reference Solution

ColumnXtimate® C18, 4.6×250 mm,5 μm (P/N: 00101-21043)
Mobile PhaseTetrabutyl ammonium hydrosulphate buffer (take 8.5g tetrabutyl ammonium hydrosulphate, dissolved in 80 mL water, add 3.4ml phosphoric acid, adjust pH value to 7.55 with 10 mol/L sodium hydroxide solution)/water/acetonitrile= (20/880/100) (mixed)
Detection220 nm
Flow Rate1.0 mL/min
Injection Volume100 µL

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