Reference Solution
| Column | Xtimate® C18, 4.6×250 mm,5 μm (P/N: 00101-21043) |
| Mobile Phase | Tetrabutyl ammonium hydrosulphate buffer (take 8.5g tetrabutyl ammonium hydrosulphate, dissolved in 80 mL water, add 3.4ml phosphoric acid, adjust pH value to 7.55 with 10 mol/L sodium hydroxide solution)/water/acetonitrile= (20/880/100) (mixed) |
| Detection | 220 nm |
| Temperature | 25℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 100 µL |
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