Raw material
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Mobile Phase | 6.8g monopotassium phosphate, 0.5g Sodium pentanesulfonate dissolved in 1000mL water, adjust pH to 2.5 with Phosphoric Acid |
| Detection | 215 nm |
| Temperature | 35 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
| Note | Transition with 100%water before use and back flush after use |
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