Raw material

ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm      P/N: 00207-31043
Mobile Phase6.8g monopotassium phosphate, 0.5g Sodium pentanesulfonate dissolved in 1000mL water, adjust pH to 2.5 with Phosphoric Acid
Detection215 nm
Temperature35 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl
NoteTransition with 100%water before use and back flush after use

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