Tandex LH-20 was prepared by hydroxypropylation of Tandex G-25, suitable for the separation of lipotropic molecules in organic solvents, the purification of natural products in organic solvents, such as steroids, terpenes, lipids, and low-molecular-weight peptides.
The separation principle of Tandex LH-20 mainly has two aspects: gel filtration and distribution of reversed phase (in the reversed-phase solvent). Because of the gel filtration, compounds of large molecules which have weak retention ability are eluted first, and compounds of small molecules which have weak retention ability come out of the column later. If reversed-phase solvent is used to elute, Tandex LH-20 has reversed-phase distribution for compounds. Compounds with strong polarity and weak retention ability will be flushed off first, and Compounds with weak polarity and strong retention ability will be flushed later. If normal phase solvent is used to elute, they are mainly separated by gel filtration.
Common solvents of mobile phase are: water, methanol, acetone, ethyl acetate, dichloromethane
Solvent solubility, polarity, boiling point, toxicity are all need to be taken into account, dichloromethane is usually used when comparison of polarity and alkalinity between separated substances is not obvious. Methanol is usually used to separate materials with ring (including benzene ring), anddextran gel has strong adsorption on ring materials. LH-20 has both hydrophilic and lipophilic properties, and the polarity of the separated material plays an important role during the separation process.
|Bead size of dry power||30-120µm|
|Exclusion range (globulin)||＜5kDa|
|Max. flow rate||700cm/h|
|Storage temperature||Keep dry and dark，4-30℃|
1. Before sample loading, the sample must pass through membrane filtration and pigment remove, otherwise impurities and pigment will be adsorbed on the resin, affecting the normal use of the resin.
2. In the process of use, avoid using high concentration of strong acid and strong alkali, the concentration of acid and alkali should be less than 0.15 mole. The alkali will slow down the flow.
3. Adsorption and elution methods are different for various samples, which can be carried out according to relevant literature.
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