Assay
| Column | Xtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043) |
| Gradient Program | Disodium hydrogen phosphate buffer solution containing 0.1% tetrabutyl ammonium hydroxide (take 10% tetrabutyl ammonium hydroxide aqueous solution 8.0ml and disodium hydrogen phosphate 2.2g, dissolve them in water to 780ml, and adjust the pH value to 7.8 with phosphoric acid) /methanol= 78/22 |
| Detection | 280 nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
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