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C11H10FNO_Ultisil AQ-C18 on Xtimate C18

Column Ultisil® AQ-C18 4.6×150mm, 3μm(P/N 00207-21041)
Detection 230nm
Temperature 35°C
Flow Rate 1.0ml/min
Injection Volume 20µl

Mobile Phase

Time
(min)
50mmol/L diammonium hydrogen phosphate(pH6.0) : Acetonitrile = 95:5 50mmol/L diammonium hydrogen phosphate(pH6.0) : Acetonitrile = 95:5
0 70 30
30 40 60
40 40 60
45 0 100
55 0 100
55.1 70 30
65 70 30