| Column | Ultisil® AQ-C18 4.6×150mm, 3μm(P/N 00207-21041) |
| Detection | 230nm |
| Temperature | 35°C |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20µl |
Mobile Phase
| Time (min) | 50mmol/L diammonium hydrogen phosphate(pH6.0) : Acetonitrile = 95:5 | 50mmol/L diammonium hydrogen phosphate(pH6.0) : Acetonitrile = 95:5 |
| 0 | 70 | 30 |
| 30 | 40 | 60 |
| 40 | 40 | 60 |
| 45 | 0 | 100 |
| 55 | 0 | 100 |
| 55.1 | 70 | 30 |
| 65 | 70 | 30 |
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