ColumnWelchrom® C18, 4.6×250mm, 5μm (P/N: 00310-02043)
Mobile PhaseAcetate buffer (18 g sodium acetate, 9.8 mL glacial acetic acid, diluted with water to 1000 mL)/methanol /isopropanol =17/70/3
Detection282 nm
Flow Rate1.0 mL/min
Injection Volume10 µL
NotesThe project should be protected from light; 2. The buffer solution should be prepared again the next day.

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