Erythromycin on Xtimate C18
System suitability solution
Gradient Program
| Time(min) | A(%) | B(%) |
| 0 | 100 | 0 |
| 15 | 100 | 0 |
| 45 | 35 | 65 |
| 47 | 0 | 100 |
| 55 | 0 | 100 |
| 56 | 100 | 0 |
| 65 | 100 | 0 |
| Column | Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043) |
| Mobile Phase | Mobile Phase A: Acetonitrile/0.2 mol/L K2HPO4(pH 7.0)/water=35/5/60 Mobile Phase B: Acetonitrile/0.2 mol/L K2HPO4(pH 7.0)/water=60/5/35 |
| Detection | 210 nm |
| Temperature | 60℃ |
| Flow Rate | 1.3 mL/min |
| Injection Volume | 100 µL |
| Notes | Column temperature affects retention time |