DL-ethyl Ester on Ultisil Polar RP
3005-1603107
1.1 Full scale
Gradient Program
| Time（min） | A% | B% |
| 0 | 95 | 5 |
| 5 | 95 | 5 |
| 15 | 65 | 35 |
| 20 | 65 | 35 |
| 25 | 40 | 60 |
| Column | Welch Ultisil Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | A:Perchloric acid aqueous solution（PH2.0） B:Methanol |
| Detector | 230nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20ul |