Sample solution
Column | Ultisil XB-NH2, 3μm, 4.6×250mm (P/N 00204-21043) |
Mobile Phase | 0.02mol/L KH2PO4:Acetonitrile =27:73, adjust pH to 5.3 with 10% HCl |
Detection | 214nm |
Temperature | 30℃ |
Flow Rate | 1.5ml/min |
Injection Volume | 10ul |
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